pertpy.data.tian_2021_crispra

pertpy.data.tian_2021_crispra()

CROP-seq of 50000 neurons treated with 374 gRNAs (CRISPRa only).

For CRISPRa, 100 genes were inclued in the CROP-seq experiments. The CROP-seq libraries included 2 sgRNAs per gene plus 6 non-targeting control sgRNAs for a total of 206 sgRNAs. On Day 10 after CRISPRa neurons were infected by the CROP-seq sgRNA library, approximately 50,000 CRISPRi neurons were loaded into 10X chips with about 25,000 input cells per lane.

References

Publication: https://doi.org/10.1038/s41593-021-00862-0 Obtained from scperturb: http://projects.sanderlab.org/scperturb/

Return type:

AnnData

Returns:

AnnData object of scPerturb single-cell perturbation data