pertpy.data.replogle_2022_k562_gwps¶
- pertpy.data.replogle_2022_k562_gwps()[source]¶
K562 cells transduced with CRISPRi (day 8 after transduction).
Here, the authors used a compact, multiplexed CRISPR interference (CRISPRi) library to assay thousands of loss-of-function genetic perturbations with single-cell RNA sequencing in chronic myeloid leukemia (CML) (K562) cell lines. For the K562 day 8 genome-scale Perturb-seq experiment, library lentivirus was packaged into lentivirus in 293T cells and empirically measured in K562 cells to obtain viral titers. CRISPRi K562 cells were transduced and all expressed genes were targeted at day 8 after transduction
References
Publication: https://doi.org/10.1016/j.cell.2022.05.013 Obtained from scperturb: http://projects.sanderlab.org/scperturb/