pertpy.data.datlinger_2017¶
- pertpy.data.datlinger_2017()[source]¶
Transcriptomics measurements of 5905 Jurkat cells induced with anti-CD3 and anti-CD28 antibodies.
For CROP-seq, Jurkat cells were transduced with a gRNA library targeting high-level regulators of T cell receptor signaling and a set of transcription factors. After 10 days of antibiotic selection and expansion, cells were stimulated with anti-CD3 and anti-CD28 antibodies or left untreated. Both conditions were analyzed using CROP-seq, measuring TCR activation for each gene knockout. The dataset comprises 5,905 high-quality single-cell transcriptomes with uniquely assigned gRNAs.
References
Publication: https://www.nature.com/articles/nmeth.4177 Obtained from scperturb: http://projects.sanderlab.org/scperturb/