pertpy.data.tian_2019_ipsc¶
- pertpy.data.tian_2019_ipsc()[source]¶
Transcriptomics of 20000 iPSCs targeted by 58 sgRNAs.
The authors performed single-cell RNA sequencing of ∼20,000 iPSCs via 10x Genomics platform. Transcripts containing sgRNA sequences were further amplified to facilitate sgRNA identity assignment among a pool of 58 sgRNAs (two sgRNAs targeting 27 selected gene and four non-targeting control sgRNAs). Following sequencing, transcriptomes and sgRNA identities were mapped to individual cells. High data quality for iPSCs was evident from ∼84,000 mean reads per cell, the median number of ∼5,000 genes detected per cell and ∼15,000 cells to which a unique sgRNA could be assigned after quality control.
References
Publication: https://doi.org/10.1016/j.neuron.2019.07.014 Obtained from scperturb: http://projects.sanderlab.org/scperturb/