pertpy.data.shifrut_2018¶
- pertpy.data.shifrut_2018()[source]¶
CD8 T-cells from two donors for two conditions (SLICE and CROP-seq).
The authors developed a new method, single guide RNA (sgRNA) lentiviral infection with Cas9 protein electroporation (SLICE), and adapted it to allow for CROP-Seq in primary human T cells. They used a library of 48 sgRNA, derived from GW screens, to explore transcriptional changes downstream of CRISPR-KO. Dataset includes CD8 T cells from two donors, for two conditions: with TCR stimulation or No stimulation.
References
Publication: https://doi.org/10.1016/j.cell.2018.10.024 Obtained from scperturb: http://projects.sanderlab.org/scperturb/