pertpy.data.adamson_2016_upr_perturb_seq

pertpy.data.adamson_2016_upr_perturb_seq()

Transcriptomics measurements of 65000 cells that were subject to 91 sgRNAs targeting 82 genes.

In UPR Perturb-seq experiment, Perturb-seq was applied to a small CRISPRi library of 91 sgRNAs targeting 82 genes. sgRNAs were delivered via pooled transduction using a mixture of separately prepared lentiviruses, and ∼65,000 transcriptomes were collected in one large pooled experiment.

References

Publication: https://www.sciencedirect.com/science/article/pii/S0092867416316609?via%3Dihub Obtained from scperturb: http://projects.sanderlab.org/scperturb/

Return type:

AnnData

Returns:

AnnData object of scPerturb prepared single-cell perturbation data