pertpy.data.replogle_2022_rpe1¶

pertpy.data.replogle_2022_rpe1()[source]¶

RPE1 cells transduced with CRISPRi (day 7 after transduction).

For day 7 essential-scale Perturb-seq experiment in retinal pigment epithelial (RPE1) cell lines, library lentivirus was packaged into lentivirus in 293T cells and empirically measured in RPE1 cells to obtain viral titers. CRISPRi RPE1 cells expressing ZIM3 KRAB-dCas9-P2A-BFP were transduced. 20Q1 Cancer Dependency Map common essential genes were targeted at day 7 after transduction.

References

Publication: https://doi.org/10.1016/j.cell.2022.05.013 Obtained from scperturb: http://projects.sanderlab.org/scperturb/

Return type:: AnnData
Returns:: AnnData object of scPerturb prepared single-cell perturbation data