pertpy.data.tian_2019_day7neuron¶
- pertpy.data.tian_2019_day7neuron()[source]¶
Transcriptomes of 20000 day 7 neurons targeted by 58 gRNAs.
The authors performed single-cell RNA sequencing of ∼20,000 day 7 neurons via 10x Genomics platform. Transcripts containing sgRNA sequences were further amplified to facilitate sgRNA identity assignment among a pool of 58 sgRNAs (two sgRNAs targeting 27 selected gene and four non-targeting control sgRNAs). Following sequencing, transcriptomes and sgRNA identities were mapped to individual cells. High data quality for neurons was evident from ∼91,000 mean reads per cell, the median number of ∼4,600 genes detected per cell and ∼8,400 cells to which a unique sgRNA could be assigned after quality control.
References
Publication: https://doi.org/10.1016/j.neuron.2019.07.014 Obtained from scperturb: http://projects.sanderlab.org/scperturb/