pertpy.data.kang_2018¶
- pertpy.data.kang_2018()[source]¶
Processed multiplexing droplet-based single cell RNA-sequencing using genetic barcodes.
HiSeq 2500 data for sequencing of PBMCs from SLE patients and 2 controls. We collected 1M cells each from frozen PBMC samples that were Ficoll isolated and prepared using the 10x Single Cell instrument according to standard protocol. Samples A, B, and C were prepared on the instrument directly following thaw, while samples 2.1 and 2.2 were cultured for 6 hours with (B) or without (A) IFN-beta stimulation prior to loading on the 10x Single Cell instrument.
References
Kang, H., Subramaniam, M., Targ, S. et al. Multiplexed droplet single-cell RNA-sequencing using natural genetic variation. Nat Biotechnol 36, 89–94 (2018). https://doi.org/10.1038/nbt.4042