pertpy.data.datlinger_2021

Contents

pertpy.data.datlinger_2021#

datlinger_2021()[source]#

Transcriptomics measurements of 151788 nuclei of four cell lines.

A large-scale scifi-RNA-seq experiment was performed with 383,000 nuclei loaded into a single microfluidic channel of the Chromium system. Four human cell lines (HEK293T, Jurkat, K562 and NALM-6) were combined in an equal mixture, and technical replicates of each cell line with different preindexing (round1) barcodes were marked. This experiment resulted in 151,788 single-cell transcriptomes passing quality control.

Source paper:

Publication: https://doi.org/10.1038/s41592-021-01153-z Obtained from scperturb: http://projects.sanderlab.org/scperturb/

Return type:

AnnData

Returns:

AnnData object of scPerturb prepared single-cell perturbation data